Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, mouse brain tissue, LNCaP cells, PC-3 cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
20049-1-AP targets MPC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | brain protein 44 |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 18-20 kDa |
| GenBank accession number | NM_015415 |
| Gene Symbol | MPC2 |
| Gene ID (NCBI) | 25874 |
| RRID | AB_10665371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95563 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRP44 belongs to the UPF0041 (0-44) family. The antibody is specific to BRP44.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPC2 antibody 20049-1-AP | Download protocol |
| IHC protocol for MPC2 antibody 20049-1-AP | Download protocol |
| IF protocol for MPC2 antibody 20049-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos. | ||
Mol Cancer 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants. | ||
J Biol Chem Mitochondrial pyruvate carrier blockade results in decreased osteoclastogenesis and bone resorption via regulating mitochondrial energy production. | ||
Hum Mol Genet Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants | ||
Life Sci Mitochondrial pyruvate carrier 2 mediates mitochondrial dysfunction and apoptosis in high glucose-treated podocytes. | ||
Brain Res Bull Downregulation of mitochondrial pyruvate carrier 2 aggravates neuronal injury in the cortex following cerebral ischemia in rat. |