Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24785-1-AP targets BRD9 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20427 Product name: Recombinant human BRD9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 133-481 aa of BC041590 Sequence: TAVEEPVPEVVPVQVETAKKSKKPSREVISCMFEPEGNACSLTDSTAEEHVLALVEHAADEARDRINRFLPGGKMGYLKRNGDGSLLYSVVNTAEPDADEEETHPVDLSSLSSKLLPGFTTLGFKDERRNKVTFLSSATTALSMQNNSVFGDLKSDEMELLYSAYGDETGVQCALSLQEFVKDAGSYSKKVVDDLLDQITGGDHSRTLFQLKQRRNVPMKPPDEAKVGDTLGDSSSSVLEFMSMKSYPDVSVDISMLSSLGKVKKELDPDDSHLNLDETTKLLQDLHEAQAERGGSRPSSNLSSLSNASERDQHHLGSPSRLSVGEQPDVTHDPYEFLQSPEPAASAKT 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 9 |
| Calculated molecular weight | 597 aa, 67 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC041590 |
| Gene Symbol | BRD9 |
| Gene ID (NCBI) | 65980 |
| RRID | AB_2879722 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8M2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription. BRD9 has six isoforms with MW 67 kDa, 23 kDa, 53 kDa, 26 kDa, 56 kDa and 61 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BRD9 antibody 24785-1-AP | Download protocol |
| WB protocol for BRD9 antibody 24785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction. | ||
Nat Commun SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors | ||
PLoS Pathog Merkel cell polyomavirus small T antigen is a viral transcription activator that is essential for viral genome maintenance | ||
J Hematol Oncol Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation | ||
Neuroscience Scalp acupuncture alleviates remote hippocampal damage in MCAO rats by inhibiting neuroinflammation: A TMT-based proteomics analysis |
