BRD4 Monoclonal antibody
BRD4 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse and More (2)
Applications
WB, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
2F7E2
Cat no : 67374-2-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
67374-2-Ig targets BRD4 in WB, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BRD4 fusion protein Ag27545 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 4 |
| Calculated molecular weight | 1362 aa, 152 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC035266 |
| Gene symbol | BRD4 |
| Gene ID (NCBI) | 23476 |
| RRID | AB_2918488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRD4 antibody 67374-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioengineered Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p. | ||
Curr Med Sci ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer |
