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BRD3 Polyclonal antibody
BRD3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11859-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, COLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 24 publications below |
| IHC | See 1 publications below |
Product Information
11859-1-AP targets BRD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BRD3 fusion protein Ag2433 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 3 |
| Calculated molecular weight | 556 aa, 61 kDa |
| Observed molecular weight | 95-105 kDa |
| GenBank accession number | BC032124 |
| Gene symbol | BRD3 |
| Gene ID (NCBI) | 8019 |
| RRID | AB_2065902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRD3 antibody 11859-1-AP | Download protocol |
| IHC protocol for BRD3 antibody 11859-1-AP | Download protocol |
| IF protocol for BRD3 antibody 11859-1-AP | Download protocol |
| IP protocol for BRD3 antibody 11859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. | ||
Nat Commun Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer. | ||
Nat Commun A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues. | ||
Nat Commun The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Zehua (Verified Customer) (02-06-2019) | Works well on fractionation lysates of MCF-7 cells.
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