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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12702-1-AP

Synonyms

10q23del, Activin receptor like kinase 3, ACVRLK3, ALK 3, ALK3, BMP type 1A receptor, BMPR 1A, BMPR1A, CD292, SKR5

Validation Data Gallery

Applications:
  • WB analysis of human skeletal muscle using 12702-1-AP

    WB analysis of human skeletal muscle using 12702-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12702-1-AP

    WB analysis of MCF-7 using 12702-1-AP

    WB result of BMPR1A antibody (12702-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMPR1A transfected MCF-7 cells.

  • WB analysis of mouse liver using 12702-1-AP

    WB analysis of mouse liver using 12702-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 12702-1-AP

    WB analysis of human heart using 12702-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 12702-1-AP

    WB analysis of K-562 using 12702-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12702-1-AP

    WB analysis of HEK-293 using 12702-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12702-1-AP

    IP experiment of HEK-293 using 12702-1-AP

    IP result of anti-BMPR1A (IP:12702-1-AP, 4ug; Detection:12702-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • IF Staining of U2OS using 12702-1-AP

    IF Staining of U2OS using 12702-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using BMPR1A antibody (12702-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HEK-293 cells, MCF-7 cells, K-562 cells, mouse liver tissue, human heart tissue
Positive IP detected inHEK-293 cells
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12702-1-AP targets BMPR1A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMPR1A fusion protein Ag3410 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type IA
Calculated molecular weight 532 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC028383
Gene symbol BMPR1A
Gene ID (NCBI) 657
RRIDAB_2064273
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BMPR1A antibody 12702-1-APDownload protocol
IF protocol for BMPR1A antibody 12702-1-APDownload protocol
IP protocol for BMPR1A antibody 12702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.

Authors - Ling Guo
View Article
humanIP

Sci Signal

Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells.

Authors - Sreeharsha Gurrapu
View Article
mouseWB,IF

Front Cell Dev Biol

Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.

Authors - Chunlei Shao
View Article
mouseWB

PLoS One

Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro.

Authors - Shasha Zhu
View Article
humanWB

Biochim Biophys Acta Gen Subj

Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels

Authors - Tianmiao Huang
View Article
ratWB

Sci Rep

Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.

Authors - Yan Zeng
View Article