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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BLVRA Monoclonal antibody

BLVRA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10F2

Cat no : 68175-1-Ig

Synonyms

Biliverdin IX alpha reductase, biliverdin reductase A, BLVR, BLVRA, BVR, BVR A, BVRA

Validation Data Gallery

Applications:
  • WB analysis using 68175-1-Ig

    WB analysis using 68175-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68175-1-Ig (BLVRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inL02 cells, DU 145 cells, U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68175-1-Ig targets BLVRA in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BLVRA fusion protein Ag32730 相同性解析による交差性が予測される生物種
Full Name biliverdin reductase A
Calculated molecular weight33.2 kDa
Observed molecular weight 37-40 kDa, 66 kDa
GenBank accession numberBC008456
Gene symbol BLVRA
Gene ID (NCBI) 644
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLVRA(Biliverdin reductase A) is also named BLVR, BVR, and belongs to the biliverdin reductase subfamily. It catalyzes the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. This protein can exist as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

Protocols

Product Specific Protocols
WB protocol for BLVRA antibody 68175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols