Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, mouse brain tissue, rat brain tissue, RAW 264.7 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
Product Information
19687-1-AP targets BLOC1S1-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | biogenesis of lysosomal organelles complex-1, subunit 1 |
| Calculated molecular weight | 17 kDa |
| Observed molecular weight | 14-17 kDa |
| GenBank accession number | NM_001487 |
| Gene Symbol | BLOC1S1 |
| Gene ID (NCBI) | 2647 |
| RRID | AB_10666440 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78537 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BLOC1S1, also named as BLOS1, GCN5L1 and RT14, belongs to the BLOC1S1 family. BLOC1S1 may be involved in the biogenesis of specialized organelles of the endosomal-lysosomal system. The antibody is specific to NLOC1S1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BLOC1S1-Specific antibody 19687-1-AP | Download protocol |
| IP protocol for BLOC1S1-Specific antibody 19687-1-AP | Download protocol |
| WB protocol for BLOC1S1-Specific antibody 19687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α | ||
EMBO J Snapin promotes HIV-1 transmission from dendritic cells by dampening TLR8 signaling. | ||
Autophagy HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. | ||
Oxid Med Cell Longev General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease. | ||
FEBS J A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems. |
