Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of mouse skeletal muscle using 10022-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10022-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10022-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10022-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10022-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 10022-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 10022-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse pancreas using 10022-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10022-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 10022-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 10022-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 10022-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using cIAP1 antibody (10022-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10022-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10022-1-AP (cIAP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 10022-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug cIAP1 Polyclonal antibody (10022-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

cIAP1 Polyclonal antibody

cIAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10022-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Synonyms

API1, BIRC2, MIHB, RNF48, Baculoviral IAP repeat-containing protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, mouse testis tissue
Positive IHC detected in	human testis tissue, mouse pancreas tissue, mouse testis tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 18 publications below
IHC	See 3 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

10022-1-AP targets cIAP1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15203 Product name: Recombinant human cIAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC016174 Sequence: MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQG 相同性解析による交差性が予測される生物種
Full Name	baculoviral IAP repeat-containing 2
Calculated molecular weight	618 aa, 70 kDa
Observed molecular weight	70 kDa, 55-60 kDa
GenBank accession number	BC016174
Gene Symbol	cIAP1
Gene ID (NCBI)	329
RRID	AB_2063790
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13490
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.

Protocols

Product Specific Protocols
FC protocol for cIAP1 antibody 10022-1-AP	Download protocol
IF protocol for cIAP1 antibody 10022-1-AP	Download protocol
IHC protocol for cIAP1 antibody 10022-1-AP	Download protocol
WB protocol for cIAP1 antibody 10022-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
zebrafish	WB,IF	Nat Genet Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis. Authors - Santoro Massimo M MM KD Validated View Article
human	IF	Mol Cell Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB. Authors - Mingjian Du View Article
human	WB	Mol Cell A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation. Authors - Michael Hinz KD Validated View Article
mouse	WB	Acta Pharm Sin B A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein. Authors - Ziqiao Yuan View Article
human	WB	Dev Cell Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform. Authors - Maria Zachari View Article
mouse	WB	J Immunol Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling. Authors - Peters Anna L AL View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skeletal muscle using 10022-1-AP

WB analysis of mouse liver using 10022-1-AP

WB analysis of mouse brain using 10022-1-AP

WB analysis of Jurkat using 10022-1-AP

WB analysis of mouse testis using 10022-1-AP

IHC staining of human testis using 10022-1-AP

IHC staining of human testis using 10022-1-AP

IHC staining of mouse pancreas using 10022-1-AP

IHC staining of mouse testis using 10022-1-AP

IHC staining of human pancreas using 10022-1-AP

IHC staining of human pancreas using 10022-1-AP

IF Staining of HepG2 using 10022-1-AP

IF Staining of HepG2 using 10022-1-AP

FC experiment of HepG2 using 10022-1-AP

cIAP1 Polyclonal antibody

cIAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.

A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.