BHLHE41 Polyclonal antibody
BHLHE41 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, chIP, ELISA
Conjugate
Unconjugated
Cat no : 12688-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
12688-1-AP targets BHLHE41 in WB, IP, IF, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BHLHE41 fusion protein Ag3381 相同性解析による交差性が予測される生物種 |
| Full Name | basic helix-loop-helix family, member e41 |
| Calculated molecular weight | 482 aa, 51 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC025968 |
| Gene symbol | BHLHE41 |
| Gene ID (NCBI) | 79365 |
| RRID | AB_2065361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia. | ||
FASEB J Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin. | ||
Biochem Biophys Res Commun Loss of circadian protein TIMELESS accelerates the progression of cellular senescence. | ||
J Exp Clin Cancer Res DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas. | ||
J Exp Clin Cancer Res Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma. | ||
Cell Death Dis Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells. |
