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BDNF 抗体 9 文献

Mouse Monoclonal| カタログ番号: 66292-1-Ig |クローン番号: 1E5C5

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適用アプリケーション

アプリケーション:
WB, IF, ELISA
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BDNF Antibody WB U-251 cells 66292-1-Ig

U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

BDNF Antibody IF PC-12 cells 66292-1-Ig

Immunofluorescent analysis of (10% Formaldehyde ) fixed PC-12 cells using 66292-1-Ig(BDNF antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

種特異性:
human, mouse, rat, zebrafish
WB ポジティブ:
U-251 cells, fetal human brain tissue
IF ポジティブ:
PC-12 cells
推奨希釈倍率:
WB : 1:1000-1:4000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG2a
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
BDNF fusion protein Ag11329
正式名:
brain-derived neurotrophic factor
計算分子量:
247 aa, 28 kDa
検出分子量:
32 kDa
GenBank accession number:
BC029795
Gene ID (NCBI):
627
遺伝子シンボル:
BDNF
別名:
Abrineurin, BDNF

背景説明

BDNF is a member of the family of neurotrophic factors. It was first explored and purified from pig brain in 1982, and other members of the neurotrophin family were successively discovered. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. It also plays a role in the regulation of stress response and in the biology of mood disorders. The BDNF gene is rather complex in its structure and it can express five different mRNA isoforms by alternative splicing, producing 28-37 kDa proteins.


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