BCLAF1 Monoclonal antibody
BCLAF1 Monoclonal Antibody for WB, IF, FC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D6G7
Cat no : 67860-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, K-562 cells, HeLa cells, Jurkat cells, HEK-293 cells |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
67860-1-Ig targets BCLAF1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BCLAF1 fusion protein Ag25210 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2-associated transcription factor 1 |
| Calculated molecular weight | 920 aa, 106 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC132780 |
| Gene symbol | BCLAF1 |
| Gene ID (NCBI) | 9774 |
| RRID | AB_2918618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BCLAF1 antibody 67860-1-Ig | Download protocol |
| IF protocol for BCLAF1 antibody 67860-1-Ig | Download protocol |
| FC protocol for BCLAF1 antibody 67860-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells. | ||
Cell Mol Life Sci BCLAF1 binds SPOP to stabilize PD-L1 and promotes the development and immune escape of hepatocellular carcinoma
|
