Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 11740-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11740-1-AP
WB result of BCL7B antibody (11740-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL7B transfected HeLa cells.
WB analysis of human brain using 11740-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11740-1-AP (BCL7B antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 11740-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11740-1-AP (BCL7B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

BCL7B Polyclonal antibody

BCL7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11740-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

B cell CLL/lymphoma 7B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

11740-1-AP targets BCL7B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2333 Product name: Recombinant human BCL7B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC000956 Sequence: MSGRSVRAETRSRAKDDIKKVMAAIEKVRKWEKKWVTVGDTSLRIFKWVPVTDSKEKEKSKSNSSAAREPNGFPSDASANSSLLLEFQDENSNQSSVSDVYQLKVDSSTNSSPSPQQSESLSPAHTSDFRTDDSQPPTLGQEILEEPSLPSSEVADEPPTLTKEEPVPLETQVVEEEEDSGAPPLKRFCVDQPTVPQTASES 相同性解析による交差性が予測される生物種
Full Name	B-cell CLL/lymphoma 7B
Calculated molecular weight	22 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC000956
Gene Symbol	BCL7B
Gene ID (NCBI)	9275
RRID	AB_2063596
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BQE9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for BCL7B antibody 11740-1-AP	Download protocol
IHC protocol for BCL7B antibody 11740-1-AP	Download protocol
WB protocol for BCL7B antibody 11740-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res Structure of the nucleosome-bound human BCL7A Authors - Franck Martin View Article
human	WB	Oxid Med Cell Longev Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro. Authors - Daowen Li View Article
mouse	WB	Aging (Albany NY) S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway. Authors - Zhenxuan Shao View Article
mouse	WB,IHC	Sci Rep The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function. Authors - Lena Wischhof KO Validated View Article
human	WB,CoIP	Life Sci Alliance BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression Authors - Nicholas Dietrich KD Validated View Article
human,mouse		Exp Hematol Bcl7b and Bcl7c Subunits of BAF Chromatin Remodeling Complexes are Largely Dispensable for Hematopoiesis Authors - Pierre Priam View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11740-1-AP

WB analysis of HeLa using 11740-1-AP

WB analysis of human brain using 11740-1-AP

IHC staining of human colon cancer using 11740-1-AP

IHC staining of human colon cancer using 11740-1-AP

IHC staining of human lung cancer using 11740-1-AP

IHC staining of human lung cancer using 11740-1-AP

IHC staining of human pancreas using 11740-1-AP

IHC staining of human pancreas using 11740-1-AP

IHC staining of human colon cancer using 11740-1-AP

IF Staining of HeLa using 11740-1-AP

BCL7B Polyclonal antibody

BCL7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Structure of the nucleosome-bound human BCL7A

Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro.

S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway.

The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function.

BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression

Bcl7b and Bcl7c Subunits of BAF Chromatin Remodeling Complexes are Largely Dispensable for Hematopoiesis