BAG3 Monoclonal antibody
BAG3 Monoclonal Antibody for WB, IF, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rabbit, pig, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4G6C2
Cat no : 68076-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, LNCaP cells, rabbit heart tissue, HeLa cells, pig heart, rat heart |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
68076-1-Ig targets BAG3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rabbit, pig, rat samples.
| Tested Reactivity | Human, mouse, rabbit, pig, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BAG3 fusion protein Ag17608 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2-associated athanogene 3 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 74 kDa |
| GenBank accession number | BC006418 |
| Gene symbol | BAG3 |
| Gene ID (NCBI) | 9531 |
| RRID | AB_2918814 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG3 antibody 68076-1-Ig | Download protocol |
| IF protocol for BAG3 antibody 68076-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Physiol Biochem BAG3 protects chondrocytes against lumbar facet joint osteoarthritis by regulating autophagy and apoptosis. | ||
J Physiol Sci High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle |
