AuthentiKine™ Human beta-NGF ELISA Kit0 Publications

カタログ番号: KE00174

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
AuthentiKine™ Human beta-NGF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
3.9-250 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 95% 73%-119%
Cell culture supernatants 119% 100%-129%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 69.6 1.6 2.3
2 20 140.4 1.8 1.3
3 20 242.1 4.9 2.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 72.0 2.6 3.6
2 24 140.8 3.4 2.4
3 24 242.9 6.9 2.9

製品概要
KE00174 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The beta-NGF ELISA kit is to be used to detect and quantify protein levels of endogenous beta -NGF. The assay recognizes human beta-NGF. An antibody specific for beta -NGF has been pre-coated onto the microwells. The beta-NGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for beta-NGF is added to detect the captured beta-NGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF
背景説明

NGF is a member of the neurotrophin family that regulates cell proliferation and differentiation within specific neural tissues, under both physiological and pathological condition. As NGF plays a key role in the proliferation, differentiation, and regulation of neural stem cells, several studies have indicated the clinical potential of NGF treatment in the regeneration of nerve cells following injury. NGF is initially in a 7S, 130-kDa complex of 3 proteins: alpha-NGF, beta-NGF, and gamma-NGF, while the beta subunit, exhibits all the biological activities ascribed to NGF. Beta-NGF stimulates the growth of sympathetic and some sensory neurites.


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