Alpha Sarcoglycan Monoclonal antibody

Name: Alpha Sarcoglycan Monoclonal antibody
Brand: Proteintech
SKU: 67078-1-Ig

Alpha Sarcoglycan Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B10H7

Review Product

Cat no : 67078-1-Ig

Synonyms

50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA

Validation Data Gallery

Applications:

AllWBIHCIF

WB analysis using 67078-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 67078-1-Ig
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 67078-1-Ig
human heart tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 67078-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of mouse skeletal muscle using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of human liver using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of human liver using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of mouse heart using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of mouse heart using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of mouse tongue using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of mouse tongue using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of rat heart using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of rat heart using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of rat skeletal muscle using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IHC staining of rat skeletal muscle using 67078-1-Ig
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SGCA antibody included
IF Staining of mouse heart using 67078-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10 H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse heart using 67078-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10 H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse skeletal muscle using 67078-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10 H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse skeletal muscle using 67078-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10 H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Cat No. 67078-1-Ig

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human skeletal muscle tissue, human heart tissue, pig heart tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue
Positive IHC detected in	mouse skeletal muscle tissue, human liver tissue, mouse heart tissue, mouse tongue tissue, rat heart tissue, rat skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	mouse heart tissue, mouse skeletal muscle tissue

Planning an IHC experiment? We recommend our IHCeasy SGCA Ready-To-Use IHC Kit. SGCA primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67078-1-Ig targets Alpha Sarcoglycan in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity	Human, mouse, rat, pig
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Alpha Sarcoglycan fusion protein Ag28800 相同性解析による交差性が予測される生物種
Full Name	sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
Calculated molecular weight	387 aa, 43 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC025702
Gene symbol	SGCA
Gene ID (NCBI)	6442
RRID	AB_2882386
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Alpha Sarcoglycan antibody 67078-1-Ig	Download protocol
IHC protocol for Alpha Sarcoglycan antibody 67078-1-Ig	Download protocol
IF protocol for Alpha Sarcoglycan antibody 67078-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha Sarcoglycan Monoclonal antibody

Alpha Sarcoglycan Monoclonal Antibody for WB, IF, IHC, ELISA

Validation Data Gallery

WB analysis using 67078-1-Ig

WB analysis of human skeletal muscle using 67078-1-Ig

WB analysis of human heart using 67078-1-Ig

WB analysis using 67078-1-Ig

IHC staining of mouse skeletal muscle using 67078-1-Ig

IHC staining of mouse skeletal muscle using 67078-1-Ig

IHC staining of human liver using 67078-1-Ig

IHC staining of human liver using 67078-1-Ig

IHC staining of mouse heart using 67078-1-Ig

IHC staining of mouse heart using 67078-1-Ig

IHC staining of mouse tongue using 67078-1-Ig