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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha B Crystallin Monoclonal antibody

Alpha B Crystallin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B4C3

Cat no : 68001-1-Ig

Synonyms

Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component

Validation Data Gallery

Applications:
  • WB analysis of rat eye using 68001-1-Ig

    WB analysis of rat eye using 68001-1-Ig

    rat eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse eye using 68001-1-Ig

    WB analysis of mouse eye using 68001-1-Ig

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 68001-1-Ig

    IHC staining of mouse eye using 68001-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CRYAB antibody included
  • IHC staining of mouse eye using 68001-1-Ig

    IHC staining of mouse eye using 68001-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CRYAB antibody included

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CRYAB Ready-To-Use IHC Kit. CRYAB primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68001-1-Ig targets Alpha B Crystallin in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha B Crystallin fusion protein Ag8656 相同性解析による交差性が予測される生物種
Full Name crystallin, alpha B
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight20-22 kDa
GenBank accession numberBC007008
Gene symbol CRYAB
Gene ID (NCBI) 1410
RRIDAB_2918749
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.

Protocols

Product Specific Protocols
WB protocol for Alpha B Crystallin antibody 68001-1-IgDownload protocol
IHC protocol for Alpha B Crystallin antibody 68001-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols