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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12406

Synonyms

ACO1, Aconitase, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1

Validation Data Gallery

Applications:
  • IF Staining of mouse liver using CL488-12406

    IF Staining of mouse liver using CL488-12406

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Aconitase 1 antibody (CL488-12406) at dilution of 1:200.

  • IF Staining of mouse liver using CL488-12406

    IF Staining of mouse liver using CL488-12406

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Aconitase 1 antibody (CL488-12406) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse liver tissue
For other applications, we recommend the unconjugated version of this antibody, 12406-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-12406 targets Aconitase 1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aconitase 1 fusion protein Ag3083 相同性解析による交差性が予測される生物種
Full Name aconitase 1, soluble
Calculated molecular weight 889 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC018103
Gene symbol ACO1
Gene ID (NCBI) 48
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Aconitase 1 antibody CL488-12406Download protocol
Standard Protocols
Click here to view our Standard Protocols