Antizyme inhibitor 1 Polyclonal antibody
Antizyme inhibitor 1 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11548-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, NCI-H1299 cells, PC-3 cells |
| Positive IHC detected in | human gliomas tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
11548-1-AP targets Antizyme inhibitor 1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Antizyme inhibitor 1 fusion protein Ag2082 相同性解析による交差性が予測される生物種 |
| Full Name | antizyme inhibitor 1 |
| Calculated molecular weight | 49.5 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC019279 |
| Gene symbol | AZIN1 |
| Gene ID (NCBI) | 51582 |
| RRID | AB_2290243 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AZIN1(Antizyme inhibitor 1) is also named as OAZI, OAZIN and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine in the first and apparently rate-limiting step in polyamine biosynthesis, and playing a role in the regulation of polyamine synthesis (negative regulator of cellular polyamines).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Antizyme inhibitor 1 antibody 11548-1-AP | Download protocol |
| IHC protocol for Antizyme inhibitor 1 antibody 11548-1-AP | Download protocol |
| IF protocol for Antizyme inhibitor 1 antibody 11548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers. | ||
J Clin Invest Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma. | ||
Mol Ther Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer. | ||
Kidney Int The microRNA miR-433 promotes renal fibrosis by amplifying the TGF-β/Smad3-Azin1 pathway. | ||
FASEB J The rational discovery of multipurpose inhibitors of the ornithine decarboxylase. |
