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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AXL Monoclonal antibody

AXL Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3D12F11

Cat no : 68108-1-Ig

Synonyms

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO

Validation Data Gallery

Applications:
  • WB analysis using 68108-1-Ig

    WB analysis using 68108-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68108-1-Ig (AXL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • FC experiment of HeLa using 68108-1-Ig

    FC experiment of HeLa using 68108-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human AXL (68108-1-Ig, Clone:3D12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inNCI-H1299 cells, A549 cells, U2OS cells, A431 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

68108-1-Ig targets AXL in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AXL fusion protein Ag14401 相同性解析による交差性が予測される生物種
Full Name AXL receptor tyrosine kinase
Calculated molecular weight 894 aa, 98 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC032229
Gene symbol AXL
Gene ID (NCBI) 558
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Protocols

Product Specific Protocols
WB protocol for AXL antibody 68108-1-IgDownload protocol
FC protocol for AXL antibody 68108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols