Various lysates were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HEK-293 (WT and ATXN3 KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 13505-1-AP incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

WB result of Ataxin-3/ATXN3 antibody (13505-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ataxin-3/ATXN3 transfected HEK-293 cells.

mouse heart tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HEK-293 lysates prepared and IP of ATXN3 performed using 1.0 μg of 13505-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.