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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATPBD4 Monoclonal antibody

ATPBD4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B12E1

Cat no : 68397-1-Ig

Synonyms

ATP binding domain 4, ATPBD4, DPH6

Validation Data Gallery

Applications:
  • WB analysis using 68397-1-Ig

    WB analysis using 68397-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of K-562 using 68397-1-Ig

    WB analysis of K-562 using 68397-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, K-562 cells, LNCaP cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68397-1-Ig targets ATPBD4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATPBD4 fusion protein Ag33218 相同性解析による交差性が予測される生物種
Full Name ATP binding domain 4
Calculated molecular weight 267 aa, 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC008485
Gene symbol ATPBD4
Gene ID (NCBI) 89978
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

Protocols

Product Specific Protocols
WB protocol for ATPBD4 antibody 68397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols