Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, human kidney tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
16143-1-AP targets ATP6V1G1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9217 Product name: Recombinant human ATP6V1G1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-118 aa of BC008452 Sequence: MASQSQGIQQLLQAEKRAAEKVSEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKAKEAAALGSRGSCSTEVEKETQEKMTILQTYFRQNRDEVLDNLLAFVCDIRPEIHENYRING 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 |
| Calculated molecular weight | 118 aa, 14 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC008452 |
| Gene Symbol | ATP6V1G1 |
| Gene ID (NCBI) | 9550 |
| RRID | AB_2062686 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75348 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| IHC protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| IP protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| WB protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells. | ||
PLoS One Acidification of endothelial Weibel-Palade bodies is mediated by the vacuolar-type H+-ATPase. | ||
EBioMedicine A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.
| ||
EBioMedicine Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo. | ||
Autophagy Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate |
