Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 17115-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17115-1-AP
WB result of ATP6V1A antibody (17115-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells.
WB analysis of mouse kidney using 17115-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 17115-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 17115-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17115-1-AP
IP result of anti-ATP6V1A (IP:17115-1-AP, 4ug; Detection:17115-1-AP 1:500) with HeLa cells lysate 4000ug.
IHC staining of human pancreas using 17115-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas using 17115-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human thyroid cancer using 17115-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 17115-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 17115-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP6V1A antibody (17115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of NIH/3T3 using 17115-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ATP6V1A antibody (17115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

ATP6V1A Polyclonal antibody

ATP6V1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17115-1-AP

Publications(45)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ATP6A1, ATP6V1A1, EC:7.1.2.2, VA68, V-ATPase 69 kDa subunit

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (45)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, human placenta tissue, mouse kidney tissue, HeLa cells, Daudi cells, HepG2 cells, K-562 cells, U-87 MG cells, mouse testis tissue, rat testis tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas tissue, human thyroid cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 33 publications below
IHC	See 2 publications below
IF	See 14 publications below
IP	See 2 publications below

Product Information

17115-1-AP targets ATP6V1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10801 Product name: Recombinant human ATP6V1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 268-617 aa of BC013138 Sequence: NSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED 相同性解析による交差性が予測される生物種
Full Name	ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight	617 aa, 68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC013138
Gene Symbol	ATP6V1A
Gene ID (NCBI)	523
RRID	AB_2290195
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P38606
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Protocols

Product Specific Protocols
IF protocol for ATP6V1A antibody 17115-1-AP	Download protocol
IHC protocol for ATP6V1A antibody 17115-1-AP	Download protocol
IP protocol for ATP6V1A antibody 17115-1-AP	Download protocol
WB protocol for ATP6V1A antibody 17115-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells. Authors - Zharko Daniloski View Article
mouse	WB	Nat Immunol Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Authors - Rui Martins View Article
human	WB	Nat Microbiol The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Authors - Tomas Doyle View Article
human	WB	Autophagy Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency Authors - Fangyang Wang View Article
mouse,human	WB	Mol Psychiatry Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease. Authors - A Pérez-Cañamás View Article
human	WB	Mol Ther Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Authors - Benfu Zhong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17115-1-AP

WB analysis of HeLa using 17115-1-AP

WB analysis of mouse kidney using 17115-1-AP

WB analysis using 17115-1-AP

WB analysis of human placenta using 17115-1-AP

IP experiment of HeLa using 17115-1-AP

IHC staining of human pancreas using 17115-1-AP

IHC staining of human pancreas using 17115-1-AP

IHC staining of human thyroid cancer using 17115-1-AP

IHC staining of human thyroid cancer using 17115-1-AP

IF Staining of HeLa using 17115-1-AP

IF Staining of NIH/3T3 using 17115-1-AP

ATP6V1A Polyclonal antibody

ATP6V1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency

Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.