Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse liver tissue, rat pancreas tissue, mouse pancreas tissue, rat stomach tissue, mouse salivary gland tissue, human pancreas cancer tissue, rat salivary gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 13 publications below |
| CoIP | See 1 publications below |
Product Information
16244-1-AP targets ATP13A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9174 Product name: Recombinant human ATP13A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 615-758 aa of BC009302 Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase type 13A1 |
| Calculated molecular weight | 976 aa, 109 kDa |
| Observed molecular weight | 120-130 kDa |
| GenBank accession number | BC009302 |
| Gene Symbol | ATP13A1 |
| Gene ID (NCBI) | 57130 |
| RRID | AB_2290293 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| IHC protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| IP protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| WB protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
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Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology | ||
Mol Cell An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins
| ||
Mol Cell ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins | ||
Adv Sci (Weinh) The Endoplasmic Reticulum ATP13A1 is Essential for MAVS-Mediated Antiviral Innate Immunity
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