Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16921-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16921-1-AP
WB result of ATL3 antibody (16921-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected HeLa cells.
WB analysis using 16921-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.
WB analysis of SMMC-7721 using 16921-1-AP
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16921-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16921-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16921-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16921-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16921-1-AP
IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:5000) with HeLa cells lysate 1720 ug.
IP experiment of HeLa using 16921-1-AP
IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:8000) with HeLa cells lysate 1760 ug.
IHC staining of human stomach cancer using 16921-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16921-1-AP
Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 16921-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (16921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ATL3 Polyclonal antibody

ATL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16921-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:3.6.5.-, Atlastin-3, Atlastin 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, SMMC-7721 cells, Jurkat cells, NIH/3T3 cells, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 17 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

16921-1-AP targets ATL3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10279 Product name: Recombinant human ATL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC077727 Sequence: MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEAN 相同性解析による交差性が予測される生物種
Full Name	atlastin GTPase 3
Calculated molecular weight	541 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC077727
Gene Symbol	ATL3
Gene ID (NCBI)	25923
RRID	AB_2290228
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6DD88
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

Protocols

Product Specific Protocols
IF protocol for ATL3 antibody 16921-1-AP	Download protocol
IHC protocol for ATL3 antibody 16921-1-AP	Download protocol
IP protocol for ATL3 antibody 16921-1-AP	Download protocol
WB protocol for ATL3 antibody 16921-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Nature ER proteins decipher the tubulin code to regulate organelle distribution Authors - Pengli Zheng View Article
human	WB	Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis Authors - Wei Zhang View Article
human	WB	Nat Commun AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity Authors - Linliang Zhang View Article
human,mouse	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
		EMBO J Two FAM134B isoforms differentially regulate ER dynamics during myogenesis Authors - Viviana Buonomo View Article
	WB	Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein Authors - Megan L Norris View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16921-1-AP

WB analysis of HeLa using 16921-1-AP

WB analysis using 16921-1-AP

WB analysis of SMMC-7721 using 16921-1-AP

WB analysis of HEK-293 using 16921-1-AP

WB analysis of HeLa using 16921-1-AP

WB analysis of mouse liver using 16921-1-AP

WB analysis of HepG2 using 16921-1-AP

IP experiment of HeLa using 16921-1-AP

IP experiment of HeLa using 16921-1-AP

IHC staining of human stomach cancer using 16921-1-AP

IF Staining of HepG2 using 16921-1-AP

IF Staining of HeLa using 16921-1-AP

ATL3 Polyclonal antibody

ATL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ER proteins decipher the tubulin code to regulate organelle distribution

Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Two FAM134B isoforms differentially regulate ER dynamics during myogenesis

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein