Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of 3T3-L1 using 55190-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 55190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 55190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 55190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55190-1-AP
WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells.
WB analysis of mouse testis using 55190-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55190-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 55190-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55190-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55190-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 55190-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 55190-1-AP
IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IF Staining of A431 using 55190-1-AP
Immunofluorescent analysis of A431 cells using 55190-1-AP (ATGL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Featured Product
KD/KO Validated

ATGL Polyclonal antibody

ATGL Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 55190-1-AP

Publications(148)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Adipose triglyceride lipase, PNPLA2, Calcium-independent phospholipase A2-zeta, Desnutrin, EC:3.1.1.3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (148)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, 3T3-L1 cells, A431 cells, HeLa cells, human heart tissue, mouse liver tissue, mouse testis tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, rat liver tissue
Positive IP detected in	mouse testis tissue
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 134 publications below
IHC	See 9 publications below
IF	See 17 publications below
IP	See 2 publications below
ELISA	See 1 publications below
CoIP	See 2 publications below

Product Information

55190-1-AP targets ATGL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	patatin-like phospholipase domain containing 2
Calculated molecular weight	55 kDa
Observed molecular weight	45-55 kDa
GenBank accession number	NM_020376
Gene Symbol	ATGL
Gene ID (NCBI)	57104
RRID	AB_11182818
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96AD5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

Protocols

Product Specific Protocols
IP protocol for ATGL antibody 55190-1-AP	Download protocol
WB protocol for ATGL antibody 55190-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics Authors - Zhiheng Rao View Article
mouse	WB	Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling Authors - Kaixin Ding View Article
mouse	IHC	Cell Intermittent fasting triggers interorgan communication to suppress hair follicle regeneration Authors - Han Chen View Article
mouse	WB	J Hepatol Circadian Control of Hepatic Ischemia/Reperfusion Injury via HSD17B13-Mediated Autophagy in Hepatocytes Authors - Hui Wang View Article
pig	WB	Autophagy PRRSV NSP2 hijacks host lipophagy via a LIPE-PNPLA2-AMPK-MTOR axis to promote viral replication. Authors - Zhenbang Zhu KD Validated View Article
human	WB	Autophagy Lipophagy fuels phosphatidylcholine synthesis for Newcastle disease virus replication. Authors - Mengqing Yang View Article

Validation Data Gallery

WB analysis of 3T3-L1 using 55190-1-AP

WB analysis using 55190-1-AP

WB analysis using 55190-1-AP

WB analysis using 55190-1-AP

WB analysis of A431 using 55190-1-AP

WB analysis of mouse testis using 55190-1-AP

WB analysis of A431 using 55190-1-AP

WB analysis of human heart using 55190-1-AP

WB analysis of HeLa using 55190-1-AP

WB analysis of HeLa using 55190-1-AP

WB analysis of rat heart using 55190-1-AP

IP experiment of mouse testis using 55190-1-AP

IF Staining of A431 using 55190-1-AP

ATGL Polyclonal antibody

ATGL Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics

Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling

Intermittent fasting triggers interorgan communication to suppress hair follicle regeneration

Circadian Control of Hepatic Ischemia/Reperfusion Injury via HSD17B13-Mediated Autophagy in Hepatocytes

PRRSV NSP2 hijacks host lipophagy via a LIPE-PNPLA2-AMPK-MTOR axis to promote viral replication.

Lipophagy fuels phosphatidylcholine synthesis for Newcastle disease virus replication.

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