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ATGL Polyclonal antibody
ATGL Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 55190-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | 3T3-L1 cells, A431 cells, HeLa cells, mouse heart tissue, mouse testis tissue, human heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 43 publications below |
| IHC | See 4 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
55190-1-AP targets ATGL in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, chicken, rat, sheep, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | patatin-like phospholipase domain containing 2 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | NM_020376 |
| Gene symbol | PNPLA2 |
| Gene ID (NCBI) | 57104 |
| RRID | AB_11182818 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATGL antibody 55190-1-AP | Download protocol |
| IP protocol for ATGL antibody 55190-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Pineal Res Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin. | ||
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
Food Chem Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity. | ||
Biochim Biophys Acta Mol Basis Dis Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr-/- mice | ||
Int J Mol Med PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF‑R.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Li (Verified Customer) (07-12-2019) | We have tried a couple different ATGL antibodies from other vendors, but no one is working as good as we want. So we tried this one, it works great.
|
FH Shantel (Verified Customer) (05-02-2019) | Works well in Western Blot. I used mouse liver tissue, 40ug/ul protein per lane at 1:1000 dilution.
 |
