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  • KD/KO Validated

ATG2B Polyclonal antibody

ATG2B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25155-1-AP

Synonyms

ATG2B, C14orf103



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25155-1-AP targets ATG2B in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG2B fusion protein Ag18412 相同性解析による交差性が予測される生物種
Full Name ATG2 autophagy related 2 homolog B (S. cerevisiae)
Calculated molecular weight 2078 aa, 233 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC015016
Gene symbol ATG2B
Gene ID (NCBI) 55102
RRIDAB_2810874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG2B antibody 25155-1-APDownload protocol
IHC protocol for ATG2B antibody 25155-1-APDownload protocol
IF protocol for ATG2B antibody 25155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
humanWB

Nat Cell Biol

ATG9A protects the plasma membrane from programmed and incidental permeabilization.

Authors - Aurore Claude-Taupin
  • KO Validated
humanIF

Nat Commun

The autophagy protein ATG9A enables lipid mobilization from lipid droplets.

Authors - Elodie Mailler
  • KO Validated
humanWB

Autophagy

SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores.

Authors - Jee-Eun Lee
humanWB

Autophagy

Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation.

Authors - Jing-Xiang Zheng
humanWB,IF

EMBO Rep

A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Authors - Mihaela Bozic