Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, human testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Product Information
21550-1-AP targets ASZ1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16217 Product name: Recombinant human ASZ1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-372 aa of BC034963 Sequence: SEDDGWEIGYLDRTSQKLKRLLPIEEKKEKFKKAMTIGDVSLVQELLDSGISVDSNFQYGWTPLMYAASVANAELVRVLLDRGANASFEKDKQSILITACSAHGSEEQILKCVELLLSRNADPNVACRRLMTPIMYAARDGHTQVVALLVAHGAEVNTQDENGYTALTWAARQGHKNIVLKLLELGANKMLQTKDGKMPSEIAKRNKHHEIFNLLSFTLNPLEGKLQQLTKEDTICKILTTDSDREKDHIFSSYTAFGDLEVFLHGLGLEHMTDLLKERDITLRHLLTMREDEFTKNGITSKDQQKILAALKELQVEEIQFGELSEETKLEISGDEFLNFLLKLNKQCGHLIT 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat, SAM and basic leucine zipper domain containing 1 |
| Calculated molecular weight | 475 aa, 53 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC034963 |
| Gene Symbol | ASZ1 |
| Gene ID (NCBI) | 136991 |
| RRID | AB_10858622 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWH4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ASZ1 antibody 21550-1-AP | Download protocol |
| WB protocol for ASZ1 antibody 21550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. | ||
Dev Cell TDRD1 phase separation drives intermitochondrial cement assembly to promote piRNA biogenesis and fertility | ||
Development MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis | ||
Development MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis. | ||
Andrology Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes | ||
Nucleic Acids Res Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. |
