Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of COS-7 using 12331-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12331-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12331-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12331-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12331-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 12331-1-AP
IP result of anti-ASH2L (IP:12331-1-AP, 4ug; Detection:12331-1-AP 1:500) with COLO 320 cells lysate 2000ug.
IHC staining of human gliomas using 12331-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12331-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ASH2L Polyclonal antibody

ASH2L Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12331-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COS-7 cells, COLO 320 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 2 publications below
ChIP	See 1 publications below

Product Information

12331-1-AP targets ASH2L in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity	human, mouse, monkey
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3008 Product name: Recombinant human ASH2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 234-534 aa of BC015936 Sequence: HGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP 相同性解析による交差性が予測される生物種
Full Name	ash2 (absent, small, or homeotic)-like (Drosophila)
Calculated molecular weight	628 aa, 69 kDa
Observed molecular weight	80 kDa
GenBank accession number	BC015936
Gene Symbol	ASH2L
Gene ID (NCBI)	9070
RRID	AB_2059837
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBL3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for ASH2L antibody 12331-1-AP	Download protocol
IP protocol for ASH2L antibody 12331-1-AP	Download protocol
WB protocol for ASH2L antibody 12331-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation. Authors - Esteban A Orellana View Article
human	WB	Nat Commun Menin enhances c-Myc-mediated transcription to promote cancer progression. Authors - Gongwei Wu View Article
human	WB	Cell Rep Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3 Authors - Ling Ye View Article
human	WB,IHC,IF,IP,ChIP	Cancer Sci ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription. Authors - Kai Zeng KD Validated View Article
mouse	WB,IP	Mol Neurobiol WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling. Authors - Minhan Ka View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of COS-7 using 12331-1-AP

WB analysis of COLO 320 using 12331-1-AP

WB analysis of COLO 320 using 12331-1-AP

WB analysis of COLO 320 using 12331-1-AP

WB analysis of COLO 320 using 12331-1-AP

IP experiment of COLO 320 using 12331-1-AP

IHC staining of human gliomas using 12331-1-AP

IHC staining of human gliomas using 12331-1-AP

ASH2L Polyclonal antibody

ASH2L Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3

ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.

WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.