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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B3C7

Cat no : 67092-2-Ig

Synonyms

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

Validation Data Gallery

Applications:
  • WB analysis using 67092-2-Ig

    WB analysis using 67092-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

67092-2-Ig targets ASAH1 in WB, ELISA applications and shows reactivity with Human, Pig, Rat, Rabbit samples.

Tested Reactivity Human, Pig, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag28645 相同性解析による交差性が予測される生物種
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated molecular weight 44 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC016828
Gene symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for ASAH1 antibody 67092-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols