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  • KD/KO Validated

ARL8B Polyclonal antibody

ARL8B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13049-1-AP

Synonyms

ARL10C, ARL8B, Gie1



Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

13049-1-AP targets ARL8B in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, chicken, rat, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL8B fusion protein Ag3711 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 8B
Calculated molecular weight 186 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC013131
Gene symbol ARL8B
Gene ID (NCBI) 55207
RRIDAB_2059000
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Protocols

Product Specific Protocols
WB protocol for ARL8B antibody 13049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Lysosomal positioning coordinates cellular nutrient responses.

Authors - Korolchuk Viktor I VI
  • KD Validated
monkeyWB

Sci Adv

The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

Authors - Meng Lu
  • KD Validated
mouseWB,IF

Sci Adv

Endosomal sorting drives the formation of axonal prion protein endoggresomes.

Authors - Romain Chassefeyre
  • KO Validated
humanWB

Nat Commun

RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.

Authors - Tal Keren-Kaplan
mouseWB

J Cell Biol

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

Authors - Natalia Schiefermeier
mouseIF,CoIP,WB

Dev Cell

Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C.

Authors - Joseph C Roney
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Olivia (Verified Customer) (01-08-2020)

WB: Lysates from mouse bone marrow-derived macrophages were subjected to SDS PAGE followed by western blot with 13049-1-AP(Arl8b antibody) at a dilution of 1:500 overnight at 4°C.IF: Mouse bone marrow-derived macrophages were fixed in 4% PFA and permeabilized with 0.5% saponin before incubation with 1:500 Arl8b antibody (in 1% BSA/0.1% saponin/PBS) overnight at 4°C, followed by incubation with anti-rabbit Alexa Fluor 568 for 1h at room temperature.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse bone marrow-derived macrophages
ARL8B Antibody Western Blot,Immunofluorescence, validation (1:500 dilution) in Mouse bone marrow-derived macrophages (Cat no:13049-1-AP)