Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of fetal human brain using 13049-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13049-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 13049-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13049-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 13049-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13049-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13049-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13049-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 13049-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13049-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 13049-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARL8B antibody (13049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

ARL8B Polyclonal antibody

ARL8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13049-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

ADP-ribosylation factor-like protein 10C, ADP-ribosylation factor-like protein 8B, ARL10C, EC:3.6.5.2, GIE1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	fetal human brain tissue, human brain tissue, mouse brain tissue, NIH/3T3 cells
Positive IHC detected in	mouse brain tissue, human stomach cancer tissue, mouse kidney tissue, rat pancreas tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 13 publications below
WB	See 20 publications below
IHC	See 2 publications below
IF	See 12 publications below
CoIP	See 1 publications below

Product Information

13049-1-AP targets ARL8B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3711 Product name: Recombinant human ARL8B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC013131 Sequence: MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDEKQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor-like 8B
Calculated molecular weight	186 aa, 22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC013131
Gene Symbol	ARL8B
Gene ID (NCBI)	55207
RRID	AB_2059000
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NVJ2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Protocols

Product Specific Protocols
IF protocol for ARL8B antibody 13049-1-AP	Download protocol
IHC protocol for ARL8B antibody 13049-1-AP	Download protocol
WB protocol for ARL8B antibody 13049-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Lysosomal positioning coordinates cellular nutrient responses. Authors - Korolchuk Viktor I VI KD Validated View Article
human	WB	Nat Commun DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy Authors - Rahul Kumar View Article
rat	IF	Nat Commun Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons Authors - Chun Hei Li KD Validated View Article
monkey	WB	Sci Adv The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes. Authors - Meng Lu KD Validated View Article
mouse	WB,IF	Sci Adv Endosomal sorting drives the formation of axonal prion protein endoggresomes. Authors - Romain Chassefeyre KO Validated View Article
human	WB	Nat Commun RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. Authors - Tal Keren-Kaplan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of fetal human brain using 13049-1-AP

WB analysis of mouse brain using 13049-1-AP

WB analysis of NIH/3T3 using 13049-1-AP

WB analysis of human brain using 13049-1-AP

IHC staining of mouse brain using 13049-1-AP

IHC staining of human stomach cancer using 13049-1-AP

IHC staining of human stomach cancer using 13049-1-AP

IHC staining of mouse kidney using 13049-1-AP

IHC staining of rat pancreas using 13049-1-AP

IHC staining of rat stomach using 13049-1-AP

IF Staining of A549 using 13049-1-AP

ARL8B Polyclonal antibody

ARL8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Lysosomal positioning coordinates cellular nutrient responses.

DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy

Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons

The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

Endosomal sorting drives the formation of axonal prion protein endoggresomes.

RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.