Validation Data Gallery
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Tested Applications
| Positive WB detected in | HUVEC cells, human brain tissue |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 8 publications below |
| IF | See 4 publications below |
Product Information
10202-1-AP targets ARL4C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0246 Product name: Recombinant human ARL4C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-174 aa of BC001051 Sequence: MLGLDSAGKTTVLYRLKFNEFVNTVPTIGFNTEKIKLSNGTAKGISCHFWDVGGQEKLRPLWKSYSRCTDGIIYVVDSVDVDRLEEAKTELHKVTKFAENQGTPLLVIANKQDLPKSLPVAEIEKQLALHELIPATTYHVQPACAIIGEGLTEGMDKLYEMILKRRKSLKQKKK 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 4C |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21-25 kDa |
| GenBank accession number | BC001051 |
| Gene Symbol | ARL4C |
| Gene ID (NCBI) | 10123 |
| RRID | AB_2058661 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARL4C antibody 10202-1-AP | Download protocol |
| IHC protocol for ARL4C antibody 10202-1-AP | Download protocol |
| IP protocol for ARL4C antibody 10202-1-AP | Download protocol |
| WB protocol for ARL4C antibody 10202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. | ||
Genome Med Integrative epigenomic and high-throughput functional enhancer profiling reveals determinants of enhancer heterogeneity in gastric cancer.
| ||
Proc Natl Acad Sci U S A CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis.
| ||
Front Pharmacol Identification of biomarkers and potential drug targets in osteoarthritis based on bioinformatics analysis and mendelian randomization | ||
Cancer Sci ARL4C is associated with initiation and progression of lung adenocarcinoma and represents a therapeutic target. | ||
iScience Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.
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