Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, HeLa cells, mouse lung tissue, mouse spleen tissue, Y79 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 3 publications below |
Product Information
10232-1-AP targets ARL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0334 Product name: Recombinant human ARL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-184 aa of BC002530 Sequence: GLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLPGALSSNAIREVLELDSIRSHHWCIQGCSAVTGENLLPGIDWLLDDISSRIFTAD 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 2 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21-25 kDa |
| GenBank accession number | BC002530 |
| Gene Symbol | ARL2 |
| Gene ID (NCBI) | 402 |
| RRID | AB_2034885 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36404 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. ADP-ribosylation factor-like 2 (ARL2) is a member of a functionally distinct group of ARF-like genes. ARL2 interacts with the tubulin-specific chaperone protein known as cofactor D. ARL2 downregulates the tubulin GAP activity of cofactor C, D, and E, and inhibits the binding of D to native tubulin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARL2 antibody 10232-1-AP | Download protocol |
| WB protocol for ARL2 antibody 10232-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem MicroRNA-15b modulates cellular ATP levels and degenerates mitochondria via Arl2 in neonatal rat cardiac myocytes.
| ||
J Biol Chem Selective Impairment of a Subset of Ran-GTP-binding Domains of Ran-binding protein 2 (Ranbp2) Suffices to Recapitulate the Degeneration of the Retinal Pigment Epithelium (RPE) Triggered by Ranbp2 Ablation. | ||
J Cell Biochem GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis. | ||
Cell Cycle GTPase-activating protein Elmod2 is essential for meiotic progression in mouse oocytes. | ||
Invest Ophthalmol Vis Sci Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis. | ||
PeerJ Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection. |
