Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16012-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 16012-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16012-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16012-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 16012-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.
IP experiment of Neuro-2a using 16012-1-AP
IP result of anti-ARL1 (IP:16012-1-AP, 4ug; Detection:16012-1-AP 1:4000) with Neuro-2a cells lysate 2400 ug.
IHC staining of mouse brain using 16012-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16012-1-AP (ARL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 16012-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16012-1-AP (ARL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 16012-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARL1 antibody (16012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

ARL1 Polyclonal antibody

ARL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16012-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ADP ribosylation factor like 1, ADP-ribosylation factor-like protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, K-562 cells, HeLa cells, mouse liver tissue, HepG2 cells, Neuro-2a cells, C6 cells
Positive IP detected in	Neuro-2a cells
Positive IHC detected in	human stomach cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

16012-1-AP targets ARL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8770 Product name: Recombinant human ARL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC007000 Sequence: MGGFFSSIFSSLFGTREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVETVTYKNLKFQVWDLGGQTSIRPYWRCYYSNTDAVIYVVDSCDRDRIGISKSELVAMLEEEELRKAILVVFANKQDMEQAMTSSEMANSLGLPALKDRKWQIFKTSATKGTGLDEAMEWLVETLKSRQ 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor-like 1
Calculated molecular weight	181 aa, 20 kDa
Observed molecular weight	20 kDa
GenBank accession number	BC007000
Gene Symbol	ARL1
Gene ID (NCBI)	400
RRID	AB_2243131
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P40616
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for ARL1 antibody 16012-1-AP	Download protocol
IHC protocol for ARL1 antibody 16012-1-AP	Download protocol
IP protocol for ARL1 antibody 16012-1-AP	Download protocol
WB protocol for ARL1 antibody 16012-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins Authors - Huaqing Deng View Article
human	WB	Nature FSP1 is a glutathione-independent ferroptosis suppressor. Authors - Sebastian Doll View Article
human	WB,IP	Nat Protoc Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation. Authors - Wouter W Kallemeijn View Article
human	WB	Nat Commun Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins. Authors - Noura Alzahofi View Article
human	WB	J Cell Biol ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network. Authors - Morié Ishida KO Validated View Article
human	IF	Cell Rep Role for the IFT-A Complex in Selective Transport to the Primary Cilium. Authors - Wenxiang Fu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16012-1-AP

WB analysis using 16012-1-AP

WB analysis of K-562 using 16012-1-AP

WB analysis of mouse liver using 16012-1-AP

WB analysis using 16012-1-AP

IP experiment of Neuro-2a using 16012-1-AP

IHC staining of mouse brain using 16012-1-AP

IHC staining of human stomach cancer using 16012-1-AP

IF Staining of HeLa using 16012-1-AP

ARL1 Polyclonal antibody

ARL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins

FSP1 is a glutathione-independent ferroptosis suppressor.

Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation.

Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins.

ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.

Role for the IFT-A Complex in Selective Transport to the Primary Cilium.