Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 10790-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10790-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10790-1-AP
WB result of ARF1 antibody (10790-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HEK-293 cells.
WB analysis of COS-7 using 10790-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10790-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10790-1-AP
WB result of ARF1 antibody (10790-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HeLa cells.
IHC staining of human breast cancer using 10790-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10790-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10790-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10790-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10790-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10790-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10790-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10790-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (10790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10790-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10790-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ADP ribosylation factor 1, ADP-ribosylation factor 1, EC:3.6.5.2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (36)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, COS-7 cells, HEK-293 cells, HeLa cells, rat brain tissue
Positive IHC detected in	human breast cancer tissue, human prostate cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 27 publications below
IHC	See 3 publications below
IF	See 10 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

10790-1-AP targets ARF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1244 Product name: Recombinant human ARF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC009247 Sequence: MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor 1
Calculated molecular weight	21 kDa
Observed molecular weight	18-21 kDa
GenBank accession number	BC009247
Gene Symbol	ARF1
Gene ID (NCBI)	375
RRID	AB_2227609
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P84077
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment. (PMID: 7759471, PMID: 16042562).This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
IF protocol for ARF1 antibody 10790-1-AP	Download protocol
IHC protocol for ARF1 antibody 10790-1-AP	Download protocol
WB protocol for ARF1 antibody 10790-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature CLCC1 governs ER bilayer equilibration to maintain lipid homeostasis. Authors - Lingzhi Wu KO Validated View Article
human	IF	Cell Metab Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex Authors - Chenxi Zhao View Article
human	WB,IP	Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation Authors - Jian Zhou View Article
human	WB	Cell Host Microbe A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Authors - Cheryl N Miller KD Validated View Article
mouse	WB	Nat Cell Biol COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells Authors - Domhnall McHugh View Article
human	IF	Nat Commun NME3 is a gatekeeper for DRP1-dependent mitophagy in hypoxia Authors - Chih-Wei Chen View Article

Validation Data Gallery

WB analysis using 10790-1-AP

WB analysis using 10790-1-AP

WB analysis of HEK-293 using 10790-1-AP

WB analysis of COS-7 using 10790-1-AP

WB analysis of HEK-293 using 10790-1-AP

WB analysis of HeLa using 10790-1-AP

IHC staining of human breast cancer using 10790-1-AP

IHC staining of human breast cancer using 10790-1-AP

IHC staining of human colon cancer using 10790-1-AP

IHC staining of human colon cancer using 10790-1-AP

IHC staining of human prostate cancer using 10790-1-AP

IHC staining of human prostate cancer using 10790-1-AP

IF Staining of HeLa using 10790-1-AP

IF Staining of HeLa using 10790-1-AP

IF Staining of HeLa using 10790-1-AP

ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CLCC1 governs ER bilayer equilibration to maintain lipid homeostasis.

Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex

Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation

A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.

COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells

NME3 is a gatekeeper for DRP1-dependent mitophagy in hypoxia

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