Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis of HepG2 using 14427-1-AP
WB result of Apolipoprotein AI antibody (14427-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Apolipoprotein AI transfected HepG2 cells.
WB analysis using 14427-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 14427-1-AP
human blood were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human ileum using 14427-1-AP
human ileum tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14427-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 14427-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14427-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14427-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 14427-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens).
IHC staining of human lung using 14427-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 14427-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse liver using 14427-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse liver using 14427-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 14427-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 14427-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (14427-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 14427-1-AP

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

APOA1, apolipoprotein A I, APOA 1, ApoA I, ApoAI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (51)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, human brain tissue, human ileum tissue, human plasma, mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 41 publications below
IHC	See 11 publications below
IF	See 5 publications below
IP	See 2 publications below

Product Information

14427-1-AP targets Apolipoprotein AI in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5793 Product name: Recombinant human APOA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-267 aa of BC005380 Sequence: RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ 相同性解析による交差性が予測される生物種
Full Name	apolipoprotein A-I
Calculated molecular weight	31 kDa
Observed molecular weight	26-30 kDa
GenBank accession number	BC005380
Gene Symbol	APOA1
Gene ID (NCBI)	335
RRID	AB_2056524
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02647
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
FC protocol for Apolipoprotein AI antibody 14427-1-AP	Download protocol
IF protocol for Apolipoprotein AI antibody 14427-1-AP	Download protocol
IHC protocol for Apolipoprotein AI antibody 14427-1-AP	Download protocol
WB protocol for Apolipoprotein AI antibody 14427-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IP	Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. Authors - Yong-Hyun Han View Article
mouse	WB,IP	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
mouse	WB	J Control Release Lipid-mediated protein corona regulation with increased apolipoprotein A-I recruitment for glioma targeting Authors - Yiwei Zhang View Article
human	IHC	Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. Authors - Xiaohui Liu View Article
human	WB	Theranostics Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment Authors - Te Bu View Article
		Br J Pharmacol A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice. Authors - Sanhu Gou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 14427-1-AP

WB analysis using 14427-1-AP

WB analysis of human plasma using 14427-1-AP

WB analysis of human ileum using 14427-1-AP

WB analysis of human brain using 14427-1-AP

IHC staining of human liver cancer using 14427-1-AP

IHC staining of human liver cancer using 14427-1-AP

IHC staining of human liver using 14427-1-AP

IHC staining of human liver using 14427-1-AP

IHC staining of human lung using 14427-1-AP

IHC staining of human lung using 14427-1-AP

IF Staining of mouse liver using 14427-1-AP

IF Staining of mouse liver using 14427-1-AP

IF Staining of HeLa using 14427-1-AP

FC experiment of HepG2 using 14427-1-AP

Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Lipid-mediated protein corona regulation with increased apolipoprotein A-I recruitment for glioma targeting

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment

A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice.

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