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  • Featured Product
  • KD/KO Validated

AP1M2 Polyclonal antibody

AP1M2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10618-1-AP

Synonyms

AP 1 complex subunit mu 2, AP mu chain family member mu1B, AP1 mu2, AP1M2, HSMU1B, MU 1B, Mu adaptin 2, MU1B, Mu1B adaptin, mu2

Validation Data Gallery

Applications:
  • WB analysis of mouse colon using 10618-1-AP

    WB analysis of mouse colon using 10618-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Hacat cells using 10618-1-AP

    WB analysis of Hacat cells using 10618-1-AP

    WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate.

  • WB analysis of BxPC-3 using 10618-1-AP

    WB analysis of BxPC-3 using 10618-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10618-1-AP

    IHC staining of human pancreas cancer using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas cancer using 10618-1-AP

    IHC staining of human pancreas cancer using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 10618-1-AP

    IHC staining of human kidney using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 10618-1-AP

    IHC staining of human kidney using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse colon tissue, Hacat cells
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10618-1-AP targets AP1M2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP1M2 fusion protein Ag0961 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 2 subunit
Calculated molecular weight 48 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC005021
Gene symbol AP1M2
Gene ID (NCBI) 10053
RRIDAB_2242895
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Protocols

Product Specific Protocols
WB protocol for AP1M2 antibody 10618-1-APDownload protocol
IHC protocol for AP1M2 antibody 10618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols