ANGPTL4 Monoclonal antibody
ANGPTL4 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D2H6
Cat no : 67577-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, COLO 320 cells, Caco-2 cells, HT-29 cells, mouse brain tissue, A549 cells |
| Positive IF detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
67577-1-Ig targets ANGPTL4 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ANGPTL4 fusion protein Ag30070 相同性解析による交差性が予測される生物種 |
| Full Name | angiopoietin-like 4 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC023647 |
| Gene symbol | ANGPTL4 |
| Gene ID (NCBI) | 51129 |
| RRID | AB_2882790 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANGPTL4 antibody 67577-1-Ig | Download protocol |
| IF protocol for ANGPTL4 antibody 67577-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Biol Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia |
