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- KD/KO Validated
Angiopoietin 1 Polyclonal antibody
Angiopoietin 1 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 23302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A375 cells, rat heart tissue, HeLa cells, K-562 cells, C6 cells, U-87 MG cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 25 publications below |
| IF | See 2 publications below |
Product Information
23302-1-AP targets Angiopoietin 1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Angiopoietin 1 fusion protein Ag18829 相同性解析による交差性が予測される生物種 |
| Full Name | angiopoietin 1 |
| Calculated molecular weight | 498 aa, 58 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC152411 |
| Gene symbol | ANGPT1 |
| Gene ID (NCBI) | 284 |
| RRID | AB_2879250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
| IF protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomater Res High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration | ||
Mol Ther Nucleic Acids Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC. | ||
Neural Regen Res Endorepellin downregulation promotes angiogenesis after experimental traumatic brain injury | ||
J Cell Mol Med IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction. | ||
Br J Pharmacol Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis. | ||
Cell Physiol Biochem Neuregulin-1 Promotes Myocardial Angiogenesis in the Rat Model of Diabetic Cardiomyopathy. |
