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KD/KO Validated

AMPD3 Polyclonal antibody

Name: AMPD3 Polyclonal antibody
Brand: Proteintech
SKU: 23997-1-AP

AMPD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23997-1-AP

Synonyms

AMP deaminase 3, AMP deaminase isoform E, AMPD3, Erythrocyte AMP deaminase

Validation Data Gallery

Applications:

AllWBIPIHC

WB analysis using 23997-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 23997-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23997-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23997-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 23997-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 23997-1-AP
IP result of anti-AMPD3 (IP:23997-1-AP, 4ug; Detection:23997-1-AP 1:800) with mouse kidney tissue lysate 4800ug.
IHC staining of human placenta using 23997-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 23997-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 23997-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 23997-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 23997-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	RAW 264.7 cells, rat kidney tissue, HEK-293 cells, mouse kidney tissue, mouse lung tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human placenta tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 1 publications below
IP	See 2 publications below

Product Information

The immunogen of 23997-1-AP is AMPD3 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AMPD3 fusion protein Ag21199 相同性解析による交差性が予測される生物種
Full Name	adenosine monophosphate deaminase (isoform E)
Calculated molecular weight	776 aa, 90 kDa
Observed molecular weight	70 kDa, 90 kDa
GenBank accession number	BC126118
Gene symbol	AMPD3
Gene ID (NCBI)	272
RRID	AB_2879396
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for AMPD3 antibody 23997-1-AP	Download protocol
IHC protocol for AMPD3 antibody 23997-1-AP	Download protocol
IP protocol for AMPD3 antibody 23997-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB,IP	J Mol Cell Cardiol Translational regulation by miR-301b upregulates AMP deaminase in diabetic hearts. Authors - Yuki Tatekoshi View Article
rat	WB	J Mol Cell Cardiol Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload. Authors - Yusuke Igaki View Article
human,mouse	WB,IHC	Nat Metab Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease. Authors - Zeyun Mi KD Validated View Article
mouse	WB	J Med Chem Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease Authors - Bowen Yang View Article
rat	WB,IP	Physiol Rep Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts Authors - Toshifumi Ogawa View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPD3 Polyclonal antibody

AMPD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 23997-1-AP

WB analysis of mouse lung using 23997-1-AP

WB analysis of rat kidney using 23997-1-AP

WB analysis of HEK-293 using 23997-1-AP

WB analysis of mouse kidney using 23997-1-AP

IP experiment of mouse kidney using 23997-1-AP

IHC staining of human placenta using 23997-1-AP

IHC staining of human placenta using 23997-1-AP

IHC staining of human colon using 23997-1-AP

IHC staining of human colon using 23997-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Translational regulation by miR-301b upregulates AMP deaminase in diabetic hearts.

Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload.

Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease.

Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease

Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts