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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ALKBH5 Recombinant antibody

ALKBH5 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2K9

Cat no : 82083-1-RR

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 82083-1-RR

    WB analysis of HeLa using 82083-1-RR

    WB result of ALKBH5 antibody (82083-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells.

  • WB analysis using 82083-1-RR

    WB analysis using 82083-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82083-1-RR (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 82083-1-RR

    IF Staining of U-251 using 82083-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ALKBH5 antibody (82083-1-RR, Clone: 2K9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, A549 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IF detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

82083-1-RR targets ALKBH5 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALKBH5 fusion protein Ag10516 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated molecular weight 458 aa, 51 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC062339
Gene symbol ALKBH5
Gene ID (NCBI) 54890
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH5 antibody 82083-1-RRDownload protocol
IF protocol for ALKBH5 antibody 82083-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols