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KD/KO Validated

ALDH4A1 Polyclonal antibody

Name: ALDH4A1 Polyclonal antibody
Brand: Proteintech
SKU: 11604-1-AP

ALDH4A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11604-1-AP

Synonyms

ALDH4, ALDH4A1, P5C dehydrogenase, P5CD, P5CDh, P5CDhL, P5CDhS

Validation Data Gallery

Applications:

AllWBIPIHC

WB analysis of human heart using 11604-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11604-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11604-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 11604-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11604-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11604-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 11604-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11604-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11604-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11604-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 11604-1-AP
IP result of anti-ALDH4 A1 (IP:11604-1-AP, 4ug; Detection:11604-1-AP 1:800) with mouse skeletal muscle tissue lysate 3600ug.
IHC staining of human liver cancer using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 11604-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4 A1 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 11604-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human heart tissue, HepG2 cells, human brain tissue, human liver tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, mouse small intestine tissue, rat heart tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

11604-1-AP targets ALDH4A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALDH4A1 fusion protein Ag2147 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 4 family, member A1
Calculated molecular weight	563 aa, 62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC023600
Gene symbol	ALDH4A1
Gene ID (NCBI)	8659
RRID	AB_2258046
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).

Protocols

Product Specific Protocols
WB protocol for ALDH4A1 antibody 11604-1-AP	Download protocol
IHC protocol for ALDH4A1 antibody 11604-1-AP	Download protocol
IP protocol for ALDH4A1 antibody 11604-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC. Authors - Liu Wei W KD Validated View Article
human	WB	Sci Rep Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure. Authors - Bobby Jones View Article
human,mouse	WB,IHC	J Exp Clin Cancer Res Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer. Authors - Chaoqun Liu View Article
mouse	WB	Nat Metab Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells Authors - Samantha J Linder KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH4A1 Polyclonal antibody

ALDH4A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of human heart using 11604-1-AP

WB analysis of human liver using 11604-1-AP

WB analysis of human skeletal muscle using 11604-1-AP

WB analysis of mouse small intestine using 11604-1-AP

WB analysis of HepG2 using 11604-1-AP

WB analysis of human brain using 11604-1-AP

WB analysis of rat heart using 11604-1-AP

WB analysis of K-562 using 11604-1-AP

WB analysis of human heart using 11604-1-AP

WB analysis of mouse skeletal muscle using 11604-1-AP

IP experiment of mouse skeletal muscle using 11604-1-AP

IHC staining of human liver cancer using 11604-1-AP

IHC staining of human liver cancer using 11604-1-AP

IHC staining of human liver cancer using 11604-1-AP

IHC staining of human liver cancer using 11604-1-AP

IHC staining of human prostate cancer using 11604-1-AP

IHC staining of human liver using 11604-1-AP

IHC staining of human liver using 11604-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.

Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells