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AKT Polyclonal antibody
AKT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10176-2-AP
Validation Data Gallery
|Positive WB detected in
|A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
|Positive IP detected in
|Positive IHC detected in
|human ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, mouse brain tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Sample-dependent, check data in validation data gallery
10176-2-AP targets AKT in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, goat, chicken, rat, zebra finches, mouse, fish, Zebrafish, hamster, pig
|Host / Isotype
|Rabbit / IgG
|AKT fusion protein Ag0213 相同性解析による交差性が予測される生物種
|v-akt murine thymoma viral oncogene homolog 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
|Product Specific Protocols
|WB protocol for AKT antibody 10176-2-AP
|IHC protocol for AKT antibody 10176-2-AP
|IF protocol for AKT antibody 10176-2-AP
|IP protocol for AKT antibody 10176-2-AP
|FC protocol for AKT antibody 10176-2-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hyopil (Verified Customer) (08-23-2021)
Worked well with western blotting
Azita (Verified Customer) (06-02-2021)
Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.