Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 21 publications below |
| IHC | See 9 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
11889-1-AP targets AGGF1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, macaque, rhesus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2497 Product name: Recombinant human AGGF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-270 aa of BC029382 Sequence: DMEHTLRIPEVGVSKFHAEIYFDHDLQSYVPVDQGSQNGTIVNGKQILQPKTKCDPYVLEHGDEVKIGETVLSFHIHPGSDTCDGCEPGQVRAHLRLDKKDESFVGPTLSKEEKELERRKELKKIRVKYGLQNTEYEDEKTLKNPKYKDRAGKRREQVGSEGTFQRDDAPASVHSEITDSNKGRKMLEKMGWKKGEGLGKDGGGMKTPIQLQLRRTHAGLGTGKPSSFEDVHLLQNKNKKNWDKARERFTENFPETKPQKDDPGTMP 相同性解析による交差性が予測される生物種 |
| Full Name | angiogenic factor with G patch and FHA domains 1 |
| Calculated molecular weight | 714 aa, 81 kDa |
| Observed molecular weight | 84-100 kDa |
| GenBank accession number | BC029382 |
| Gene Symbol | AGGF1 |
| Gene ID (NCBI) | 55109 |
| RRID | AB_2305338 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N302 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AGGF1 antibody 11889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling
| ||
Nat Commun AGGF1 therapy inhibits thoracic aortic aneurysms by enhancing integrin α7-mediated inhibition of TGF-β1 maturation and ERK1/2 signaling | ||
Nat Commun A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure. | ||
Mol Ther The 3' Untranslated Region Protects the Heart from Angiotensin II-Induced Cardiac Dysfunction via AGGF1 Expression. | ||
Arterioscler. Thromb. Vasc. Biol. Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.
| ||
Arterioscler Thromb Vasc Biol Aggf1 Specifies Hemangioblasts at the Top of Regulatory Hierarchy via Npas4l and mTOR-S6K-Emp2-ERK Signaling
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