AFP Polyclonal antibody
AFP Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14550-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, HuH-7 cells, human placenta tissue, L02 cells, HepG2cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 28 publications below |
| IHC | See 39 publications below |
| IF | See 31 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
14550-1-AP targets AFP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種 |
| Full Name | alpha-fetoprotein |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 68-72 kDa |
| GenBank accession number | BC027881 |
| Gene symbol | AFP |
| Gene ID (NCBI) | 174 |
| RRID | AB_2223933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AFP antibody 14550-1-AP | Download protocol |
| IHC protocol for AFP antibody 14550-1-AP | Download protocol |
| IF protocol for AFP antibody 14550-1-AP | Download protocol |
| IP protocol for AFP antibody 14550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes. | ||
J Clin Invest Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment. | ||
J Clin Invest HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis. | ||
Nat Commun Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma. | ||
Nat Commun Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets. |
