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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADRB2 Polyclonal antibody

ADRB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29864-1-AP

Synonyms

ADRB2, ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor, Beta 2 adrenoceptor, Beta 2 adrenoreceptor, BETA2AR

Validation Data Gallery

Applications:
  • WB analysis using 29864-1-AP

    WB analysis using 29864-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 29864-1-AP (ADRB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 29864-1-AP

    WB analysis using 29864-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 29864-1-AP (ADRB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

29864-1-AP targets ADRB2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRB2 fusion protein Ag32313 相同性解析による交差性が予測される生物種
Full Name adrenergic, beta-2-, receptor, surface
Calculated molecular weight46 kDa
Observed molecular weight46-48 kDa
GenBank accession numberBC012481
Gene symbol ADRB2
Gene ID (NCBI) 154
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity, and type 2 diabetes. This antibody detects ADRB2 with a molecular weight of 46-48 kDa as has been shown by some researches (PMID: 30542705; 35075132).

Protocols

Product Specific Protocols
WB protocol for ADRB2 antibody 29864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

iScience

Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration

Authors - Hao-Kun Xu
View Article
humanWB

Oncol Lett

β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways

Authors - Xingcheng Lin
View Article