Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, PC-12 cells, T-47D cells, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 16 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
14266-1-AP targets ADRA2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5562 Product name: Recombinant human ADRA2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-377 aa of BC050414 Sequence: QIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTPAAGPGEERVGAAKASRWRGRQNREKRFTFVLA 相同性解析による交差性が予測される生物種 |
| Full Name | adrenergic, alpha-2A-, receptor |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC050414 |
| Gene Symbol | ADRA2A |
| Gene ID (NCBI) | 150 |
| RRID | AB_2636822 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08913 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADRA2A (Adrenoceptor Alpha-2) belongs to the adrenoceptor alpha-2 family of GPCRs coupled to the inhibitory G-alpha subunit (Gi) that suppresses adenylyl cyclase activity, thereby suppressing the cyclization of AMP to cAMP (PMID: 38132444). ADRA2A inhibits the flow of sympathetic nerves in the central nervous system and plays a major role in sedation, anxiolysis, and antinociception (PMID: 33915198).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADRA2A antibody 14266-1-AP | Download protocol |
| WB protocol for ADRA2A antibody 14266-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission. | ||
Nat Chem Biol Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A | ||
Sci Adv Sympathetic nerve aggravates autoimmune skin disease via NE-adrenergic receptor axis: Neuroimmune cross-talk insights from vitiligo. | ||
Biomed Pharmacother Dexmedetomidine facilitates autophagic flux to promote liver regeneration by suppressing GSK3β activity in mouse partial hepatectomy | ||
Front Immunol β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis. | ||
Food Funct Folic acid enhances intestinal stem cell-mediated epithelial regeneration depending on β-adrenergic receptor signaling. |