Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 21613-1-AP
Human plasma was subjected to SDS PAGE followed by western blot with 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21613-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 21613-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of rat brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 40x lens).
IF Staining of 3T3-L1 using 21613-1-AP
Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using Adiponectin antibody (21613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of NIH/3T3 using 21613-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Adiponectin antibody (21613-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Adiponectin Polyclonal antibody

Adiponectin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21613-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADIPOQ, apM-1, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (43)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human plasma, NIH/3T3 cells, 3T3-L1 cells
Positive IHC detected in	rat brown adipose tissue, human placenta tissue, human prostate cancer tissue, human skin tissue, mouse brown adipose tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	3T3-L1 cells, NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 30 publications below
IHC	See 3 publications below
IF	See 10 publications below

Product Information

21613-1-AP targets Adiponectin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, sheep, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16304 Product name: Recombinant human ADIPOQ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC096308 Sequence: MLLLGAVLLLLALPGHDQETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN 相同性解析による交差性が予測される生物種
Full Name	adiponectin, C1Q and collagen domain containing
Calculated molecular weight	244 aa, 26 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC096308
Gene Symbol	Adiponectin
Gene ID (NCBI)	9370
RRID	AB_2878891
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15848
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
IF protocol for Adiponectin antibody 21613-1-AP	Download protocol
IHC protocol for Adiponectin antibody 21613-1-AP	Download protocol
WB protocol for Adiponectin antibody 21613-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	IF	Nature Inhibition of IL-11 signalling extends mammalian healthspan and lifespan Authors - Anissa A Widjaja View Article
mouse	IF	Nat Commun FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination Authors - Chuxiang Lei View Article
mouse	WB	Nat Commun Adipocyte small extracellular vesicle-derived microRNA-30a-3p exacerbates hepatic steatosis in high fat diet-fed male mice. Authors - Tian Zhang View Article
		Acta Pharm Sin B A novel NAMPT activator ameliorates obesity by repressing ACSL1-dependent lipid synthesis. Authors - Zhi Jiang View Article
mouse	WB	Cell Rep Med Exosome-based bone-targeting drug delivery alleviates impaired osteoblastic bone formation and bone loss in inflammatory bowel diseases Authors - Jiawei Guo View Article
mouse	IHC	Cell Mol Biol Lett Single-cell RNA sequencing identifies inherent abnormalities of adipose-derived stem cells from nonlesional sites of patients with localized scleroderma Authors - Xuanyu Liu View Article

Validation Data Gallery

WB analysis using 21613-1-AP

WB analysis of NIH/3T3 using 21613-1-AP

WB analysis of 3T3-L1 using 21613-1-AP

IHC staining of rat brown adipose using 21613-1-AP

IHC staining of rat brown adipose using 21613-1-AP

IHC staining of human placenta using 21613-1-AP

IHC staining of human placenta using 21613-1-AP

IHC staining of human prostate cancer using 21613-1-AP

IHC staining of human prostate cancer using 21613-1-AP

IHC staining of mouse skeletal muscle using 21613-1-AP

IHC staining of mouse skeletal muscle using 21613-1-AP

IHC staining of mouse brown adipose using 21613-1-AP

IHC staining of mouse brown adipose using 21613-1-AP

IHC staining of human skin using 21613-1-AP

IHC staining of human skin using 21613-1-AP

IF Staining of 3T3-L1 using 21613-1-AP

IF Staining of NIH/3T3 using 21613-1-AP

Adiponectin Polyclonal antibody

Adiponectin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of IL-11 signalling extends mammalian healthspan and lifespan

FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination

Adipocyte small extracellular vesicle-derived microRNA-30a-3p exacerbates hepatic steatosis in high fat diet-fed male mice.

A novel NAMPT activator ameliorates obesity by repressing ACSL1-dependent lipid synthesis.

Exosome-based bone-targeting drug delivery alleviates impaired osteoblastic bone formation and bone loss in inflammatory bowel diseases

Single-cell RNA sequencing identifies inherent abnormalities of adipose-derived stem cells from nonlesional sites of patients with localized scleroderma

Able™

Able^™