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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Gamma Adducin Polyclonal antibody (17585-1-AP)

    WB analysis of NIH/3T3 using 17585-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Gamma Adducin Polyclonal antibody (17585-1-AP)

    WB analysis of HEK-293 using 17585-1-AP

    WB result of Gamma Adducin antibody (17585-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Gamma Adducin transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse kidney tissue using Gamma Adducin Polyclonal antibody (17585-1-AP)

    WB analysis of mouse kidney using 17585-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Gamma Adducin Polyclonal antibody (17585-1-AP)

    WB analysis of K-562 using 17585-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using Gamma Adducin Polyclonal antibody (17585-1-AP)

    IP experiment of NIH/3T3 using 17585-1-AP

    IP result of anti-Gamma Adducin (IP:17585-1-AP, 4ug; Detection:17585-1-AP 1:500) with NIH/3T3 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Gamma Adducin Polyclonal antibody (17585-1-AP)

    IHC staining of human stomach cancer using 17585-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17585-1-AP (Gamma Adducin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Gamma Adducin Polyclonal antibody (17585-1-AP)

    IHC staining of mouse kidney using 17585-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17585-1-AP (Gamma Adducin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Gamma Adducin Polyclonal antibody (17585-1-AP)

    IHC staining of rat kidney using 17585-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17585-1-AP (Gamma Adducin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using Gamma Adducin Polyclonal antibody (17585-1-AP)

    IHC staining of rat stomach using 17585-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 17585-1-AP (Gamma Adducin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using Gamma Adducin Polyclonal antibody (17585-1-AP)

    IHC staining of rat stomach using 17585-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 17585-1-AP (Gamma Adducin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

Gamma Adducin Polyclonal antibody

Gamma Adducin Polyclonal Antibody for WB, IP, ELISA
Cat No. 17585-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ADD3, ADD 3, ADDL, adducin 3 (gamma), Adducin-like protein 70

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells, K-562 cells, mouse kidney tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman stomach cancer tissue, mouse kidney tissue, rat kidney tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17585-1-AP targets Gamma Adducin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11318

Product name: Recombinant human ADD3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 364-707 aa of BC062559

Sequence: WKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTVPLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQFVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEGELEEYKRTIERKQQGLEDAEQELLSDDASSVSQIQSQTQSPQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITIKSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA

相同性解析による交差性が予測される生物種
Full Name adducin 3 (gamma)
Calculated molecular weight 76 kDa, 79 kDa
Observed molecular weight 80 kDa, 90 kDa
GenBank accession numberBC062559
Gene Symbol Gamma Adducin
Gene ID (NCBI) 120
RRIDAB_2223579
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UEY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Gamma Adducin antibody 17585-1-APDownload protocol
IP protocol for Gamma Adducin antibody 17585-1-APDownload protocol
WB protocol for Gamma Adducin antibody 17585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Genom

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders

Authors - Greta Pintacuda
View Article
humanWB

Stem Cell Res Ther

Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis

Authors - Hewei Xiong
  • KD Validated
View Article
humanWB

J Mol Cell Biol

QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.

Authors - Jin-Zhu Wang
View Article
humanWB

PLoS One

Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients.

Authors - Esther N Pesciotta
View Article
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