Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human heart tissue, human heart and human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2500-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
66125-1-Ig targets ACTC1-specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | actin, alpha, cardiac muscle 1 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | NM_005159 |
| Gene Symbol | ACTC1 |
| Gene ID (NCBI) | 70 |
| RRID | AB_2881524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68032 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTC1-specific antibody 66125-1-Ig | Download protocol |
| IHC protocol for ACTC1-specific antibody 66125-1-Ig | Download protocol |
| IF protocol for ACTC1-specific antibody 66125-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function | ||
J Am Heart Assoc Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion. | ||
Front Mol Biosci Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer. | ||
Immunol Res The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy. | ||
J Clin Invest Frameshift variants in C10orf71 cause dilated cardiomyopathy in human, mouse, and organoid models |